Daniel Collins PhD Defense
About the event
Daniel Collins (Garner Group)
PhD Defense
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DEVELOPMENT OF A SITE-SPECIFIC GLYCOLIGATION TO SYNTHESIZE RECOMBINANT N-GLYCOPROTEINS VIA THIOACID CAPTURE LIGATION
The goal of the project was to synthesize asparagine-linked glycoproteins with a natural linkage. We described an aspartyl thioester unnatural amino acid and its incorporation into a recombinant protein. Its utility was demonstrated in two publications by native chemical ligation. Attempts to transform the aspartyl thioester into an asparagine-linked glycoprotein with a natural linkage were unsuccessful. Hydrolysis was observed.
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